A normalised scale for structural genomics target ranking: The OB-Score

被引：51

作者：

Overton, Ian M. ^{[1
]}

Barton, Geoffrey J. ^{[1
]}

机构：

[1] Univ Dundee, Sch Life Sci, Dundee DD1 5EH, Scotland

来源：

FEBS LETTERS | 2006年 / 580卷 / 16期

基金：

英国生物技术与生命科学研究理事会;

关键词：

HTPX; structural genomics; target selection; Pfam; bioinformatics; PROTEIN-CRYSTALLIZATION; THROUGHPUT; SELECTION; DATABASE; STRATEGIES; BIOLOGY; CDD;

D O I：

10.1016/j.febslet.2006.06.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydroppobicity values for nonredundant PDB entries solved to <= 3.0 angstrom against a background of UniRef50. A highly significant difference was found between the OB-Scores for TargetDB test datasets. A wide range of OR-Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain >= 1 member with a high OB-Score, presenting favourable candidates for structural studies. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：4005 / 4009

页数：5

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