Probing of antibody-antigen reactions at electropolymerized polyaniline immunosensors using impedance spectroscopy

被引：22

作者：

Miao, YQ

Guan, JG ^{[1
]}

机构：

[1] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China

[2] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua, Peoples R China

来源：

ANALYTICAL LETTERS | 2004年 / 37卷 / 06期

关键词：

biosensor; impedance; immunosensor; polyaniline;

D O I：

10.1081/AL-120034052

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical impedance immunosensor was developed for the monitoring of intermolecular interactions between anti-human-AFP monoclonal IgG and alpha-fetoprotein (AFP). Antibody (Ab) was immobilized on the polyanifine (PAN) modified electrodes by cyclic voltammetry. The modification of electrode surface and the interaction between antibody and antigen were studied by impedance spectroscopy. The resulting immunosensor has a linear dynamic range of 200 - 800 ng/mL of AFP.

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收藏

页码：1053 / 1062

页数：10

相关论文

共 22 条

[1]

BACHIR S, 2000, SENSOR ACTUAT B-CHEM, V62, P35

[2] Polypyrrole-hydrogel composites for the construction of clinically important biosensors [J].

Brahim, S ;

Narinesingh, D ;

Guiseppi-Elie, A .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (1-2) :53-59

[3] Biomolecule immobilization on electrode surfaces by entrapment or attachment to electrochemically polymerized films. A review [J].

Cosnier, S .

BIOSENSORS & BIOELECTRONICS, 1999, 14 (05) :443-456

[4]

DOINA B, 1998, CROTICA CHEM ACTA, V71, P745

[5] ENZYME ENTRAPMENT IN ELECTRICALLY CONDUCTING POLYMERS - IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLYPYRROLE AND ITS APPLICATION IN AMPEROMETRIC GLUCOSE SENSORS [J].

FOULDS, NC ;

LOWE, CR .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1986, 82 :1259-1264

[6] Amperometric glucose biosensors based on Prussian Blue- and polyaniline-glucose oxidase modified electrodes [J].

Garjonyte, R ;

Malinauskas, A .

BIOSENSORS & BIOELECTRONICS, 2000, 15 (9-10) :445-451

[7] Application of conducting polymers to biosensors [J].

Gerard, M ;

Chaubey, A ;

Malhotra, BD .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (05) :345-359

[8] Chronopotentiometry and Faradaic impedance spectroscopy as methods for signal transduction in immunosensors [J].

Katz, E ;

Alfonta, L ;

Willner, I .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 76 (1-3) :134-141

[9] Probing of bioaffinity interactions at interfaces using impedance spectroscopy and chronopotentiometry [J].

Kharitonov, AB ;

Alfonta, L ;

Katz, E ;

Willner, I .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2000, 487 (02) :133-141

[10] Conductimetric membrane strip immunosensor with polyaniline-bound gold colloids as signal generator [J].

Kim, JH ;

Cho, JH ;

Cha, GS ;

Lee, CW ;

Kim, HB ;

Paek, SH .

BIOSENSORS & BIOELECTRONICS, 2000, 14 (12) :907-915