Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM

被引：22

作者：

McGinty, J. ^{[1
]}

Requejo-Isidro, J. ^{[1
,3
]}

Munro, I. ^{[1
]}

Talbot, C. B. ^{[1
]}

Kellett, P. A. ^{[2
]}

Hares, J. D. ^{[2
]}

Dunsby, C. ^{[1
]}

Neil, M. A. A. ^{[1
]}

French, P. M. W. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Blackett Lab, Photon Grp, London SW7 2BW, England

[2] Kentech Instruments Ltd, Wallingford OX10 8BA, Oxon, England

[3] CSIC UPV EHU, Unidad Biofis, E-48940 Leioa, Vizcaya, Spain

来源：

JOURNAL OF PHYSICS D-APPLIED PHYSICS | 2009年 / 42卷 / 13期

关键词：

LIFETIME IMAGING MICROSCOPY; REAL-TIME; SPATIAL-RESOLUTION; FREQUENCY-DOMAIN; FLUORESCENCE; SPEED; EXCITATION; ACQUISITION; DETECTOR; SYSTEM;

D O I：

10.1088/0022-3727/42/13/135103

中图分类号：

O59 [应用物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

Time-gated imaging using gated optical intensifiers provides a means to realize high speed fluorescence lifetime imaging (FLIM) for the study of fast events and for high throughput imaging. We present a signal-to-noise characterization of CCD-coupled micro-channel plate gated intensifiers used with this technique and determine the optimal acquisition parameters (intensifier gain voltage, CCD integration time and frame averaging) for measuring mono-exponential fluorescence lifetimes in the shortest image acquisition time for a given signal flux. We explore the use of unequal CCD integration times for different gate delays and show that this can improve the lifetime accuracy for a given total acquisition time.

引用

页数：9

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