Taenia solium and Taenia ovis:: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

被引：27

作者：

Gauci, Charles G.

Verastegui, Manuela R.

Gilman, Robert H.

Lightowlers, Marshall W.

机构：

[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia

[2] Univ Peruana Cayetano Heredia, Fac Ciencias, Dept Microbiol, Lima 31, Peru

[3] Johns Hopkins Sch Hyg & Publ Hlth, Dept Int Hlth, Baltimore, MD USA

来源：

EXPERIMENTAL PARASITOLOGY | 2006年 / 113卷 / 04期

基金：

英国惠康基金;

关键词：

Taenia solium; Taenia ovis; Taenia saginanta; Echinococcus granulosus; cestode; cysticercosis; parasite; vaccine; gene; oncosphere; expression; PCR; RT-PCR; cDNA; DNA; mRNA;

D O I：

10.1016/j.exppara.2006.01.006

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：272 / 275

页数：4

共 27 条

[1] CLONING AND SEQUENCING OF 2 ACTIN GENES FROM TAENIA-SOLIUM (CESTODA) [J].