Size-Dependent Accumulation of PEGylated Silane-Coated Magnetic Iron Oxide Nanoparticles in Murine Tumors

被引：226

作者：

Larsen, Esben K. U. ^{[1
,2
,3
,4
]}

Nielsen, Thomas ^{[5
,6
]}

Wittenborn, Thomas ^{[5
,6
]}

Birkedal, Henrik ^{[1
,2
,3
,4
]}

Vorup-Jensen, Thomas ^{[1
,2
,3
,4
]}

Jakobsen, Mogens H. ^{[7
]}

Ostergaard, Leif ^{[5
,6
]}

Horsman, Michael R. ^{[5
,6
]}

Besenbacher, Flemming ^{[1
,2
,3
,4
]}

Howard, Kenneth A. ^{[1
,2
,3
,4
]}

Kjems, Jorgen ^{[1
,2
,3
,4
]}

机构：

[1] Aarhus Univ, Dept Mol Biol, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Dept Phys & Astron, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[3] Aarhus Univ, Dept Chem, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[4] Aarhus Univ, Dept Med Microbiol & Immunol, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[5] Aarhus Univ Hosp, iNANO, Dept Expt Clin Oncol, DK-8000 Aarhus C, Denmark

[6] Aarhus Univ Hosp, Dept Neuroradiol, Danish Natl Res Fdn, Ctr Functionally Integrat Neurosci, DK-8000 Aarhus C, Denmark

[7] DTU Nanotech, Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

来源：

ACS NANO | 2009年 / 3卷 / 07期

关键词：

magnetic resonance imaging; cancer; magnetite nanoparticles; drug delivery; ultrasmall superparamagnetic iron oxide (USPIO) particles; polyethylene glycol; THERAPY; ANGIOGENESIS; LIPOSOMES; POLYMERS; SYSTEM;

D O I：

10.1021/nn900330m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Magnetic nanoparticles (MNP) can be used as contrast-enhancing agents to visualize tumors by magnetic resonance imaging (MRI). Here we describe an easy synthesis method of magnetic nanoparticles coated with polyethylene glycol (PEG) and demonstrate size-dependent accumulation in murine tumors following intravenous injection. Biocompatible iron oxide MNPs coated with PEG were prepared by replacing oleic acid with a biocompatible and commercially available silane-PEG to provide an easy and effective method for chemical coating. The colloidal stable PEGylated MNPs were magnetically separated into two distinct size subpopulations of 20 and 40 nm mean diameters with increased phagocytic uptake observed for the 40 nm size range in vitro. MRI detection revealed greater iron accumulation in murine tumors for 40 nm nanoparticles after intravenous injection. The enhanced MRI contrast of the larger MNPs in the tumor may be a combined result of the size-dependent extravasation and capture by macrophages in the tumor, providing important considerations for improved bioimaging approaches.

引用

页码：1947 / 1951

页数：5

共 24 条

[21] Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging [J].

Wang, YXJ ;

Hussain, SM ;

Krestin, GP .

EUROPEAN RADIOLOGY, 2001, 11 (11) :2319-2331

[22] Molecular imaging and therapy of atherosclerosis with targeted nanoparticles [J].

Wickline, Samuel A. ;

Neubauer, Anne M. ;

Winter, Patrick M. ;

Caruthers, Shelton D. ;

Lanza, Gregory M. .

JOURNAL OF MAGNETIC RESONANCE IMAGING, 2007, 25 (04) :667-680

[23] Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake [J].

Zhang, Y ;

Kohler, N ;

Zhang, MQ .

BIOMATERIALS, 2002, 23 (07) :1553-1561

[24] Size series of small indium arsenide-zinc selenide core-shell nanocrystals and their application to in vivo imaging [J].

Zimmer, JP ;

Kim, SW ;

Ohnishi, S ;

Tanaka, E ;

Frangioni, JV ;

Bawendi, MG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (08) :2526-2527

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