Reticulated vitreous carbon-based composite bienzyme electrodes for the determination of alcohols in beer samples

被引:11
作者
Peña, N [1 ]
Tárrega, R [1 ]
Reviejo, AJ [1 ]
Pingarrón, JM [1 ]
机构
[1] Univ Complutense Madrid, Dept Analyt Chem, Fac Chem, E-28040 Madrid, Spain
关键词
short-chain alcohols; bienzyme composite electrodes;
D O I
10.1081/AL-120014284
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The performance of a bienzyme amperometric composite biosensor for the determination of short-chain alcohols is reported. Alcohol oxidase and horseradish peroxidase together with the mediator ferrocene were incorporated into reticulated vitreous carbon (RVC)-epoxy resin electrode matrices. Under the optimized experimental conditions, the composite bienzyme electrode exhibited rapid amperometric responses at 0.00V to the changes in the substrate concentration. The lifetime of each single biosensor was estimated to be ca. 20 days, and reproducible responses were obtained with different electrodes constructed in the same manner.
引用
收藏
页码:1931 / 1944
页数:14
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