The Mycobacterium tuberculosis Sigma Factor σB Is Required for Full Response to Cell Envelope Stress and Hypoxia In Vitro, but It Is Dispensable for In Vivo Growth

被引：62

作者：

Fontan, P. A. ^{[1
]}

Voskuil, M. I. ^{[2
,3
]}

Gomez, M. ^{[1
]}

Tan, D. ^{[4
]}

Pardini, M. ^{[4
]}

Manganelli, R. ^{[5
]}

Fattorini, L. ^{[4
]}

Schoolnik, G. K. ^{[3
]}

Smith, I. ^{[1
]}

机构：

[1] PHRI Ctr NJMS UMDNJ, Newark, NJ 07103 USA

[2] Univ Colorado Denver, Sch Med, Dept Microbiol, Aurora, CO 80045 USA

[3] Stanford Univ, Dept Med, Div Infect Dis & Geog Med, Stanford, CA 94305 USA

[4] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy

[5] Univ Padua, Sch Med, Dept Histol Microbiol & Med Biotechnol, I-35121 Padua, Italy

来源：

JOURNAL OF BACTERIOLOGY | 2009年 / 191卷 / 18期

关键词：

GLOBAL GENE-EXPRESSION; NONREPLICATING PERSISTENCE; DIFFERENTIAL EXPRESSION; 2-COMPONENT SYSTEM; GUINEA-PIGS; VIRULENCE; MACROPHAGES; SURVIVAL; MICROARRAYS; INFECTION;

D O I：

10.1128/JB.00510-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The numerous sigma (sigma) factors present in Mycobacterium tuberculosis are indicative of the adaptability of this pathogen to different environmental conditions. In this report, we describe the M. tuberculosis sigma(B) regulon and the phenotypes of an M. tuberculosis sigB mutant strain exposed to cell envelope stress, oxidative stress, and hypoxia. The sigB mutant was especially defective in survival under hypoxic conditions in vitro, but it was not attenuated for growth in THP-1 cells or during mouse and guinea pig infection.

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收藏

页码：5628 / 5633

页数：6

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