Over-production of human interferon-γ by HCDC of recombinant Escherichia coli

被引：42

作者：

Babaeipour, V. ^{[1
]}

Shojaosadati, S. A. ^{[1
]}

Robatjazi, S. M. ^{[1
]}

Khalilzadeh, R. ^{[1
]}

Maghsoudi, N. ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Fac Engn, Dept Chem Engn, Biotechnol Grp, Tehran, Iran

来源：

PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 01期

关键词：

human interferon-gamma; fed-batch; Escherichia coli; specific growth rate; feeding strategy; high cell density cultivation (HCDC);

D O I：

10.1016/j.procbio.2006.07.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple fed-batch process was developed using a modified variable specific growth rate feeding strategy for high cell density cultivation of Escherichia coli BL21 (DE3) expressing human interferon-gamma (hIFN-gamma). The feeding rate was adjusted to achieve the maximum attainable specific growth rate during fed-batch cultivation. In this method, specific growth rate was changed from a maximum value of 0.55 h(-1) at the beginning of feeding and then it was reduced to 0.4 h(-1) at induction time. The final concentration of biomass and IFN-gamma was reached to similar to 115 g l(-1) (DCW) and 42.5 g(hIFN-gamma) l(-1) after 16.5 h, also the final specific yield and overall productivity of recombinant hIFN-gamma (rhIFN-gamma) were obtained 0.37 g(hIFN-gamma) g(-1) DCW and 2.57 g(hIFN-gamma) l(-1) h(-1), respectively. According to available data this is the highest specific yield and productivity that has been reported for recombinant proteins production yet. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：112 / 117

页数：6

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