Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase

被引：369

作者：

Sirichandra, Caroline ^{[2
]}

Gu, Dan ^{[1
]}

Hu, Heng-Cheng ^{[1
]}

Davanture, Marlene ^{[3
,4
]}

Lee, Sangmee ^{[1
]}

Djaoui, Michael ^{[2
]}

Valot, Benoit ^{[3
]}

Zivy, Michel ^{[3
]}

Leung, Jeffrey ^{[2
]}

Merlot, Sylvain ^{[2
]}

Kwak, June M. ^{[1
]}

机构：

[1] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

[2] CNRS, Inst Sci Vegetal, UPR 2355, Gif Sur Yvette, France

[3] PAPPSO, UMR Genet Vegetale, F-91190 Gif Sur Yvette, France

[4] IFR87, Gif Sur Yvette, France

来源：

FEBS LETTERS | 2009年 / 583卷 / 18期

关键词：

Abscisic acid; Guard cell; Protein kinase; Reactive oxygen species; ABSCISIC-ACID; PLANT; CA2+;

D O I：

10.1016/j.febslet.2009.08.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a similar to 40% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity.

引用

页码：2982 / 2986

页数：5

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