Validation of a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 11 (fluoro)quinolone antibiotics in swine kidney

被引：65

作者：

Toussaint, B ^{[1
]}

Bordin, G

Janosi, A

Rodriguez, AR

机构：

[1] CE, JRC, IRMM, B-2240 Geel, Belgium

[2] Univ Liege, Fac Vet Med, Lab Anal Foodstuffs Anim Origin, B-4000 Liege, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 976卷 / 1-2期

关键词：

validation; fluoroquinolones; quinolones; antibiotics;

D O I：

10.1016/S0021-9673(02)00948-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A LC-MS-MS method has been validated for the simultaneous quantification of 11 (fluoro)quinolone antibiotics at the maximum residue level (MRL) in swine kidney. The studied compounds were danofloxacine, cinoxacine, ciprofloxacine, enoxacine, enrofloxacine, flumequine, marbofloxacine, nalidixic acid, norfloxacine, ofloxacine and oxolinic acid. The method involves solid-phase extraction of these compounds followed by LC-MS-MS analysis using an electrospray ionisation interface. Limits of quantification less than or equal to50 mug/kg could be obtained in swine kidney, much lower than every MRL. The validation is discussed. This work was carried out in order to support the European Union policy on consumer health protection. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：195 / 206

页数：12

共 42 条

[1]

Aarestrup FM, 2000, VET REC, V146, P76

[2]

[Anonymous], 2003, J CHEMOMETR, DOI DOI 10.1002/cem.790

[3] Comparison between capillary electrophoresis, liquid chromatography, potentiometric and spectrophotometric techniques for evaluation of pKa values of zwitterionic drugs in acetonitrile-water mixtures [J].