Gcn5 promotes acetylation, eviction, and methylation of nucleosomes in transcribed coding regions

被引：192

作者：

Govind, Chhabi K. ^{[1
]}

Zhang, Fan ^{[1
]}

Qiu, Hongfang ^{[1
]}

Hofmeyer, Kimberly ^{[1
]}

Hinnebusch, Alan G. ^{[1
]}

机构：

[1] NICHHD, Lab Gene Regulat & Dev, Bethesda, MD 20892 USA

来源：

MOLECULAR CELL | 2007年 / 25卷 / 01期

关键词：

D O I：

10.1016/j.molcel.2006.11.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that coactivator SAGA, containing the HAT Gcn5p, occupies the GAL land ARG1 coding sequences during transcriptional induction, dependent on PIC assembly and Ser5 phosphorylation of the Pol II CTD. Induction of GAL1 increases H3 acetylation per nucleosome in the ORF, dependent on SAGA integrity but not the alternative Gcn5p-HAT complex ADA. Unexpectedly, H3 acetylation in ARG1 coding sequences does not increase during induction due to the opposing activities of multiple HDAs associated with the ORE Remarkably, inactivation of Gcn5p decreases nucleosome eviction from both GAL1 and a long (similar to 8 kb) ORF transcribed from the GAL1 promoter. This is associated with reduced Pol II occupancy at the 3' end and decreased mRNA production, selectively, for the long ORF. Gcn5p also enhances H3-K4 trimethylation in the ARG1 ORF and bulk histones. Thus, Gcn5p, most likely in SAGA, stimulates modification and eviction of nucleosomes in transcribed coding sequences and promotes Pol II elongation.

引用

页码：31 / 42

页数：12

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