Protein Nanocapsule Weaved with Enzymatically Degradable Polymeric Network

被引：139

作者：

Gu, Zhen ^{[1
,2
,3
]}

Yan, Ming ^{[1
,3
]}

Hu, Biliang ^{[5
]}

Joo, Kye-Il ^{[5
]}

Biswas, Anuradha ^{[1
,3
]}

Huang, Yu ^{[3
,4
]}

Lu, Yunfeng ^{[1
,3
]}

Wang, Pin ^{[5
]}

Tang, Yi ^{[1
,3
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Mech & Aerosp Engn, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Mat Sci & Engn, Los Angeles, CA 90095 USA

[5] Univ So Calif, Mork Family Dept Chem Engn & Mat Sci, Los Angeles, CA 90089 USA

来源：

NANO LETTERS | 2009年 / 9卷 / 12期

关键词：

INTRACELLULAR DELIVERY; THERAPEUTIC PROTEINS; LIVING CELLS; APOPTOSIS; CASPASE-3; ENZYME; ENCAPSULATION; PHOTORELEASE; HYDROGELS; PROTEASES;

D O I：

10.1021/nl902935b

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Target proteins can be functionally encapsulated using a cocoon-like polymeric nanocapsule formed by interfacial polymerization. The nanocapsule is cross-linked by peptides that can be proteolyzed by proteases upon which the protein cargo is released. The protease-mediated degradation process can be controlled in a spatiotemporal fashion through modification of the peptide cross-linker with photolabile moieties. We demonstrate the utility of this approach through the cytoplasmic delivery of the apoptosis inducing caspase-3 to cancer cells.

引用

页码：4533 / 4538

页数：6

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