DNA Origami as a Nanoscopic Ruler for Super-Resolution Microscopy

被引：222

作者：

Steinhauer, Christian ^{[3
]}

Jungmann, Ralf ^{[1
,2
]}

Sobey, Thomas L. ^{[1
,2
]}

Simmel, Friedrich C. ^{[1
,2
]}

Tinnefeld, Philip ^{[3
]}

机构：

[1] Tech Univ Munich, Phys Dept E14, D-85748 Garching, Germany

[2] Tech Univ Munich, Ctr Nanosci, D-85748 Garching, Germany

[3] Univ Munich, Ctr Nanosci, D-80799 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 47期

关键词：

DNA origami; fluorescence microscopy; molecular ruler; nanoscopic ruler; super-resolution; SINGLE; FLUORESCENCE; DYES;

D O I：

10.1002/anie.200903308

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Resolving the distance: Rectangular DNA origami labeled with fluqrophres at specific positions has been used as a nanoscopic ruler. Super-resolution microscppy based on the subsequent localization of single molecules enables two fluoropheres at a distance of about 90 nm to be optically resolved. This combination of subdiffraction imaging and DNA nano technology opens up new avenues for studying name structure and then © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8870 / 8873

页数：4

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