Antibody discovery:: phage display

被引：96

作者：

Kretzschmar, T ^{[1
]}

von Rüden, T ^{[1
]}

机构：

[1] MorphoSys AG, D-82152 Martinsried, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 06期

关键词：

D O I：

10.1016/S0958-1669(02)00380-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage display has proven to be a robust and convenient technology for the selection of high-quality human antibodies from diverse libraries. Besides enabling the identification of antibodies in a fast, high-throughput mode, which allows comprehensive protein expression analyses, phage display has been used to identify a fully human therapeutic antibody presently undergoing the regulatory process for market approval.

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页码：598 / 602

页数：5

相关论文

共 49 条

[11] Antibody arrays for high-throughput screening of antibody-antigen interactions [J].

de Wildt, RMT ;

Mundy, CR ;

Gorick, BD ;

Tomlinson, IM .

NATURE BIOTECHNOLOGY, 2000, 18 (09) :989-994

[12] Selection of large diversities of antiidiotypic antibody fragments by phage display [J].

Goletz, S ;

Christensen, PA ;

Kristensen, P ;

Blohm, D ;

Tomlinson, I ;

Winter, G ;

Karsten, U .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 315 (05) :1087-1097

[13] Strategies for selection of antibodies by phage display [J].

Griffiths, AD ;

Duncan, AR .

CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (01) :102-108

[14]

Haab BB, 2001, GENOME BIOL, V2

[15] High-throughput antibody isolation [J].

Hayhurst, A ;

Georgiou, G .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (06) :683-689

[16] Selection of cell binding and internalizing epidermal growth factor receptor antibodies from a phage display library [J].

Heitner, T ;

Moor, A ;

Garrison, JL ;

Marks, C ;

Hasan, T ;

Marks, JD .

JOURNAL OF IMMUNOLOGICAL METHODS, 2001, 248 (1-2) :17-30

[17]

Holt L J, 2000, Nucleic Acids Res, V28, pE72, DOI 10.1093/nar/28.15.e72

[18] The use of recombinant antibodies in proteomics [J].

Holt, LJ ;

Enever, C ;

de Wildt, RMT ;

Tomlinson, IM .

CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (05) :445-449

[19] BY-PASSING IMMUNIZATION - HUMAN-ANTIBODIES FROM SYNTHETIC REPERTOIRES OF GERMLINE VH GENE SEGMENTS REARRANGED INVITRO [J].

HOOGENBOOM, HR ;

WINTER, G .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (02) :381-388

[20] Designing and optimizing library selection strategies for generating high-affinity antibodies [J].

Hoogenboom, HR .

TRENDS IN BIOTECHNOLOGY, 1997, 15 (02) :62-70

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