Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2′-dipyridyldisulfide

被引：22

作者：

Glatz, Z ^{[1
]}

Maslanová, H ^{[1
]}

机构：

[1] Masaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 895卷 / 1-2期

关键词：

detection; electrophoresis; 2,2 '-dipyridyldisulfide; thiols; glutathione; sulfur compounds;

D O I：

10.1016/S0021-9673(00)00475-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2'-dipyridyldisulfide is described. 2,2'-Dipyridyldisulfide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2'-dipyridyldisulfide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 187

页数：9

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