A new support for the immobilization of penicillin acylase

被引：92

作者：

Jing, H

Li, XF

Evans, DG

Duan, X ^{[1
]}

Li, CY

机构：

[1] Beijing Univ Chem Technol, Dept Appl Chem, Beijing 100029, Peoples R China

[2] Beijing Univ Chem Technol, Dept Chem Engn, Beijing 100029, Peoples R China

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 11卷 / 01期

关键词：

MCM-41; penicillin acylase; immobilization;

D O I：

10.1016/S1381-1177(00)00218-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesoporous MCM-41 having well ordered long-range structure, large pore diameters, narrow pore-size distribution, high pore volume and specific surface area has been synthesized. The surface of MCM-41 has an abundance of weakly acidic hydroxyl groups. Assay results show that MCM-41 is a more effective support for the immobilization of Penicillin Acylase (PA) than many of other supports due to its structural and surface characteristics. PA can be immobilized on MCM-41 through either direct immobilization or covalent coupling. The former gives higher activity of IME than the later. In the direct immobilization, PA molecules are immobilized on MCM-41 through the hydrogen-bonded interaction between hydroxyl groups of MCM-41 and carbonyl or amino groups in the PA molecule. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：45 / 53

页数：9

共 12 条

[1] A NEW FAMILY OF MESOPOROUS MOLECULAR-SIEVES PREPARED WITH LIQUID-CRYSTAL TEMPLATES [J].