Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy

被引：29

作者：

Cottrell, W. J.

Wilson, J. D.

Foster, T. H.

机构：

[1] Univ Rochester, Inst Opt, Rochester, NY 14642 USA

[2] Univ Rochester, Dept Phys, Rochester, NY 14642 USA

[3] Univ Rochester, Dept Imaging Sci, Rochester, NY 14642 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 16期

关键词：

D O I：

10.1364/OL.32.002348

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present the design, construction, and initial characterization of a multifunctional imaging/scattering spectroscopy system built around a commercial inverted microscope platform. The system enables co-registered brightfield, Fourier-filtered darkfield, and fluorescence imaging; monochromatic angle-resolved scattering measurements; and white-light wavelength-resolved scattering spectroscopy from the same field of view. A fiber-based illumination system provides illumination-wavelength flexibility and a good approximation to a point source. The performance of the system in its various data acquisition modes is experimentally verified using fluorescent microspheres. This multifunctional instrument provides a platform for studies on adherent cells from which the biophysical implications of subcellular light scattering can be studied in conjunction with sensitive fluorescence-based techniques. (C) 2007 Optical Society of America.

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收藏

页码：2348 / 2350

页数：3

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