Statistical Indices for Simultaneous Large-Scale Metabolite Detections for a Single NMR Spectrum

被引：106

作者：

Chikayama, Eisuke ^{[1
]}

Sekiyama, Yasuyo ^{[1
]}

Okamoto, Mami ^{[2
]}

Nakanishi, Yumiko ^{[2
]}

Tsuboi, Yuuri ^{[3
]}

Akiyama, Kenji ^{[1
]}

Saito, Kazuki ^{[1
,4
]}

Shinozaki, Kazuo ^{[1
]}

Kikuchi, Jun ^{[1
,2
,5
]}

机构：

[1] RIKEN, Plant Sci Ctr, Metabolome Res Grp, Tsurumi Ku, Kanagawa 2300045, Japan

[2] Yokohama City Univ, Grad Sch Nanobiosci, Tsurumi Ku, Kanagawa 2300045, Japan

[3] RIKEN, Adv Sci Inst, Wako, Saitama 3510198, Japan

[4] Chiba Univ, Grad Sch Pharmaceut Sci, Inage Ku, Chiba 2638522, Japan

[5] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4640810, Japan

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

TOTAL CORRELATION SPECTROSCOPY; ARABIDOPSIS-THALIANA; METABOLOMICS; IDENTIFICATION; MIXTURES; PLANT; METABONOMICS; GENOMICS; REVEALS; SYSTEM;

D O I：

10.1021/ac9022023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

NMR-based metabolomics has become a practical and analytical methodology for discovering novel genes, biomarkers, metabolic phenotypes, and dynamic cell behaviors in organisms. Recent developments in NMR-based metabolomics, however, have not concentrated on improvements of comprehensiveness in terms of simultaneous large-scale metabolite detections. To resolve this, we have devised and implemented a statistical index, the SpinAssign p-value, in NMR-based metabolomics for large-scale metabolite annotation and publicized this information. It enables simultaneous annotation of more than 200 candidate metabolites from the single C-13-HSQC (heteronuclear single quantum coherence) NMR spectrum of a single sample of cell extract.

引用

页码：1653 / 1658

页数：6

共 55 条

[51] Database resources of the national center for biotechnology information [J].

Wheeler, David L. ;

Barrett, Tanya ;

Benson, Dennis A. ;

Bryant, Stephen H. ;

Canese, Kathi ;

Chetvernin, Vyacheslav ;

Church, Deanna M. ;

DiCuccio, Michael ;

Edgar, Ron ;

Federhen, Scott ;

Feolo, Michael ;

Geer, Lewis Y. ;

Helmberg, Wolfgang ;

Kapustin, Yuri ;

Khovayko, Oleg ;

Landsman, David ;

Lipman, David J. ;

Madden, Thomas L. ;

Maglott, Donna R. ;

Miller, Vadim ;

Ostell, James ;

Pruitt, Kim D. ;

Schuler, Gregory D. ;

Shumway, Martin ;

Sequeira, Edwin ;

Sherry, Steven T. ;

Sirotkin, Karl ;

Souvorov, Alexandre ;

Starchenko, Grigory ;

Tatusov, Roman L. ;

Tatusova, Tatiana A. ;

Wagner, Lukas ;

Yaschenko, Eugene .

NUCLEIC ACIDS RESEARCH, 2008, 36 :D13-D21

[52] Metabolomics: applications to food science and nutrition research [J].

Wishart, David S. .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2008, 19 (09) :482-493

[53] HMDB: the human metabolome database [J].

Wishart, David S. ;

Tzur, Dan ;

Knox, Craig ;

Eisner, Roman ;

Guo, An Chi ;

Young, Nelson ;

Cheng, Dean ;

Jewell, Kevin ;

Arndt, David ;

Sawhney, Summit ;

Fung, Chris ;

Nikolai, Lisa ;

Lewis, Mike ;

Coutouly, Marie-Aude ;

Forsythe, Ian ;

Tang, Peter ;

Shrivastava, Savita ;

Jeroncic, Kevin ;

Stothard, Paul ;

Amegbey, Godwin ;

Block, David ;

Hau, David D. ;

Wagner, James ;

Miniaci, Jessica ;

Clements, Melisa ;

Gebremedhin, Mulu ;

Guo, Natalie ;

Zhang, Ying ;

Duggan, Gavin E. ;

MacInnis, Glen D. ;

Weljie, Alim M. ;

Dowlatabadi, Reza ;

Bamforth, Fiona ;

Clive, Derrick ;

Greiner, Russ ;

Li, Liang ;

Marrie, Tom ;

Sykes, Brian D. ;

Vogel, Hans J. ;

Querengesser, Lori .

NUCLEIC ACIDS RESEARCH, 2007, 35 :D521-D526

[54] MetaboMiner - semi-automated identification of metabolites from 2D NMR spectra of complex biofluids [J].

Xia, Jianguo ;

Bjorndahl, Trent C. ;

Tang, Peter ;

Wishart, David S. .

BMC BIOINFORMATICS, 2008, 9 (1)

[55] Chemoselective 15N Tag for Sensitive and High-Resolution Nuclear Magnetic Resonance Profiling of the Carboxyl-Containing Metabolome [J].

Ye, Tao ;

Mo, Huaping ;

Shanaiah, Narasimhamurthy ;

Gowda, G. A. Nagana ;

Zhang, Shucha ;

Raftery, Daniel .

ANALYTICAL CHEMISTRY, 2009, 81 (12) :4882-4888

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