Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

被引：980

作者：

Cho, Won-Ki ^{[1
]}

Spille, Jan-Hendrik ^{[1
]}

Hecht, Micca ^{[1
]}

Lee, Choongman ^{[1
]}

Li, Charles ^{[2
,3
]}

Grube, Valentin ^{[1
,4
]}

Cisse, Ibrahim I. ^{[1
]}

机构：

[1] MIT, Dept Phys, Cambridge, MA 02139 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

[3] Whitehead Inst Biomed Res, Cambridge, MA 02139 USA

[4] Ludwig Maximilians Univ Munchen, Dept Phys, Geschwister Scholl Pl 1, D-80539 Munich, Germany

来源：

SCIENCE | 2018年 / 361卷 / 6400期

关键词：

SELECTIVE-INHIBITION; SUPER-ENHANCERS; HUMAN-CELLS; MICROSCOPY; LOCALIZATION; ELEMENTS; COMPLEX;

D O I：

10.1126/science.aar4199

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Poi II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator. recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.

引用

收藏

页码：412 / 415

页数：4

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