Murine fecal proteomics: A model system for the detection of potential biomarkers for colorectal cancer

被引：36

作者：

Ang, Ching-Seng ^{[2
]}

Rothacker, Julie

Patsiouras, Heather

Burgess, Antony W. ^{[2
]}

Nice, Edouard C. ^{[1
,2
]}

机构：

[1] Royal Melbourne Hosp, Prot Biosensing Lab, Ludwig Inst Canc Res, Melbourne Tumour Biol Branch, Melbourne, Vic 3050, Australia

[2] Univ Melbourne, Melbourne, Vic 3010, Australia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 19期

基金：

英国医学研究理事会;

关键词：

Murine fecal proteomics; Apc(Min/+) mice; Colorectal cancer; Biomarkers; Nano-LC-ESI-MS/MS analysis; 2-DIMENSIONAL GEL-ELECTROPHORESIS; MULTIPLE INTESTINAL NEOPLASIA; LYMPH-NODE METASTASIS; FC-GAMMA-BP; OCCULT BLOOD; BINDING-PROTEIN; COLON-CANCER; MOLECULAR-CLONING; SERUM BIOMARKERS; PLASMA PROTEOME;

D O I：

10.1016/j.chroma.2009.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC-MS/MS to analyse fecal samples from Apc(Min/+) mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin. haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3330 / 3340

页数：11

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