Porphyrins as spectroscopic sensors for conformational studies of DNA

被引：47

作者：

Balaz, Milan ^{[1
]}

Bitsch-Jensen, Klaus ^{[1
]}

Mammana, Angela ^{[1
]}

Ellestad, George A. ^{[1
]}

Nakanishi, Koji ^{[1
]}

Berova, Nina ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

PURE AND APPLIED CHEMISTRY | 2007年 / 79卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

porphyrins; circular dichroism; DNA conformation; DNA probe; B- to Z-DNA transition; oligonucleotides; DNA conjugates;

D O I：

10.1351/pac200779040801

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Molecular systems containing two or more interacting porphyrins show remarkable spectroscopic features that allow for a very sensitive detection of conformational changes on the microscale level by different methods, such as fluorescence and electronic circular dichroism (ECD). Covalent porphyrin-DNA assemblies can provide a CD profile (exciton couplet) within the porphyrin Soret band region which is very diagnostic for DNA conformational changes. Additionally, covalently linked porphyrins have been shown to function as DNA molecular caps and to stabilize the non-self-complementary non-Watson-Crick guanine-adenine DNA sequence via their strong pi-pi stacking.

引用

页码：801 / 809

页数：9

共 51 条

[1]

Ardhammar M., 2000, CIRCULAR DICHROISM P, P741, DOI DOI 10.1021/AC802626E

[2] Porphyrins conjugated to DNA as CD reporters of the salt-induced B to Z-DNA transition [J].