Multiplexed electrospray deposition for protein microarray with micromachined silicon device

Multiplexed electrospray deposition device capable of delivering picoliter volumes made by silicon micromachining technology has been developed as a deposition tool for making protein microarrays in a noncontact mode. Upon application of potential difference in the range of 7-9 kV, biomolecules dissolved in suitable buffer with nonionic surfactant and loaded on the electrospray tips were dispensed on the substrate with microfabricated hydrogel features (1-10 mu m) in cone-jet mode. Schiff base chemistry followed by reductive amination was utilized for covalent immobilization. (C) 2007 American Institute of Physics.