Functional analysis of Phe605, a conserved aromatic amino acid in squalene-hopene cyclases

被引：33

作者：

Hoshino, T ^{[1
]}

Kouda, M

Abe, T

Sato, T

机构：

[1] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Niigata 9502181, Japan

来源：

CHEMICAL COMMUNICATIONS | 2000年 / 16期

关键词：

D O I：

10.1039/b004129g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Incubation of squalene with the site-directed mutant F605A of squalene-hopene cyclase from Alicyclobacillus acidocaldarius yielded many triterpenes consisting of the 6/6/5-fused tri-, 6/6/6/5-fused tetra-, and 6/6/6/6/5-fused pentacyclic skeletons, the function of F605 being assignable for facilitating the ring expansion and for stabilizing the hopanyl C22-cation, possibly via cation-pi interactions.

引用

页码：1485 / 1486

页数：2

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