Light regulation of aptamer activity: An anti-thrombin aptamer with caged thymidine nucleobases

被引：144

作者：

Heckel, A ^{[1
]}

Mayer, G ^{[1
]}

机构：

[1] Univ Bonn, Kekule Inst Organ Chem & Biochem, D-53121 Bonn, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 03期

关键词：

D O I：

10.1021/ja043285e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

"Caged" derivatives of a 15 nucleotide ssDNA anti-thrombin aptamer have been synthesized in which thymidine nucleotides are modified with photolabile protecting groups. One caged thymidine in a key location is enough to completely mask the aptamer's function in respect to their affinity for thrombin and their inhibition of the blood clotting cascade. With light (366 nm) the caging group can be removed, yielding the unmodified active aptamer. Copyright © 2005 American Chemical Society.

引用

页码：822 / 823

页数：2

共 19 条

[1] Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos [J].

Ando, H ;

Furuta, T ;

Tsien, RY ;

Okamoto, H .

NATURE GENETICS, 2001, 28 (04) :317-325

[2] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[3] Caged RNA: photo-control of a ribozyme reaction [J].

Chaulk, SG ;

MacMillan, AM .

NUCLEIC ACIDS RESEARCH, 1998, 26 (13) :3173-3178

[4] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[5] In vivo-applied functional RNAs as tools in proteomics and genomics research [J].

Famulok, M ;

Verma, S .

TRENDS IN BIOTECHNOLOGY, 2002, 20 (11) :462-466

[6]

KROCK L, IN PRESS ANGEW CHEM

[7] A NOVEL NUCLEOTIDE-BASED THROMBIN INHIBITOR INHIBITS CLOT-BOUND THROMBIN AND REDUCES ARTERIAL PLATELET THROMBUS FORMATION [J].

LI, WX ;

KAPLAN, AV ;

GRANT, GW ;

TOOLE, JJ ;

LEUNG, LLK .

BLOOD, 1994, 83 (03) :677-682

[8]

MARRIOTT G, 1998, METHODS ENZYMOLOGY, V291

[9] Targeting expression with light using caged DNA [J].

Monroe, WT ;

McQuain, MM ;

Chang, MS ;

Alexander, JS ;

Haselton, FR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (30) :20895-20900

[10] An ambiguous structure of a DNA 15-mer thrombin complex [J].

Padmanabhan, K ;

Tulinsky, A .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1996, 52 :272-282

← 1 2 →