Genomic and morphological changes of neuroblastoma cells in response to three-dimensional matrices

被引:72
作者
Li, Grace N. [1 ]
Livi, Liane L. [1 ]
Gourd, Celinda M. [1 ]
Deweerd, Elizabeth S. [1 ]
Hoffman-Kim, Diane [1 ]
机构
[1] Brown Univ, Ctr Biomed Engn, Dept Mol Pharmacol Physiol & Biotechnol, Providence, RI 02912 USA
来源
TISSUE ENGINEERING | 2007年 / 13卷 / 05期
关键词
D O I
10.1089/ten.2006.0251
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Advances in neural tissue engineering require a comprehensive understanding of neuronal growth in 3 dimensions. This study compared the gene expression of SH-SY5Y human neuroblastoma cells cultured in 3-dimensional (3D) with those cultured in 2-dimensional (2D) environments. Microarray analysis demonstrated that, in response to varying matrix geometry, SH-SY5Y cells exhibited differential expression of 1,766 genes in collagen I, including those relevant to cytoskeleton, extracellular matrix, and neurite outgrowth. Cells extended longer neurites in 3D collagen I cultures than in 2D. Real-time reverse transcriptase polymerase chain reaction experiments andmorphological analysis comparing collagen I and Matrigel tested whether the differential growth and gene expression reflected influences of culture dimension or culture material. SH-SY5Y neuroblastoma cells responded to geometry by differentially regulating cell spreading and genes associated with actin in similar patterns for both materials; however, neurite outgrowth and the expression of the gene encoding for neurofilament varied with the type of material. Electronmicroscopy and mechanical analysis showed that collagen I was more fibrillar than Matrigel, with larger inter-fiber distance and higher stiffness. Taken together, these results suggest complex cell-material interactions, in which the dimension of the culture material influences gene expression and cell spreading and the structural and mechanical properties of the culture material influence gene expression and neurite outgrowth.
引用
收藏
页码:1035 / 1047
页数:13
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