LNA: a versatile tool for therapeutics and genomics

被引：510

作者：

Petersen, M ^{[1
]}

Wengel, J ^{[1
]}

机构：

[1] Univ So Denmark, Dept Chem, Nucl Acid Ctr, DK-5230 Odense M, Denmark

来源：

TRENDS IN BIOTECHNOLOGY | 2003年 / 21卷 / 02期

关键词：

D O I：

10.1016/S0167-7799(02)00038-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Locked nucleic acid (LNA) is a nucleic acid analogue that displays unprecedented hybridization affinity towards complementary DNA and RNA. Structural studies have shown LNA to be an RNA mimic, fitting seamlessly into an A-type duplex geometry. Several reports have revealed LNA as a most promising molecule for the development of oligonucleotide-based therapeutics. For example, Tat-dependent transcription and telomerase activity have been efficiently suppressed by LNA oligomers, and efficient cleavage of highly structured RNA has been achieved using LNA-modified DNAzymes ('LNAzyme'). Furthermore, convincing examples of the application of LNA to nucleic acid diagnostics have been reported, including high capturing efficiencies and unambiguous scoring of single-nucleotide polymorphisms.

引用

页码：74 / 81

页数：8

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