Photoelectrophoretic transport and hybridization of DNA oligonucleotides on unpatterned silicon substrates

被引:16
作者
Gurtner, C [1 ]
Edman, CF [1 ]
Formosa, RE [1 ]
Heller, MJ [1 ]
机构
[1] Nanogen Inc, San Diego, CA 92121 USA
关键词
D O I
10.1021/ja000487b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We describe a platform for photoelectrophoretic transport and electronic hybridization of fluorescence-labeled DNA oligonucleotides in a low conductivity electrolyte. At the core of the platform is a chemically stabilized semiconductor photodiode or photoconductor surface coated with a streptavidin-agarose permeation layer. Micro-illumination of this surface generates photoelectrochemical currents that are used to electrophoretically transport and attach biotinylated DNA capture strands to arbitrarily selected locations. The same process is then used to transport and electronically hybridize fluorescence-labeled DNA target strands to the previously attached capture strands. Signal detection is accomplished either by a fluorescence scanner ora CCD camera. This represents a flexible electronic DNA assay platform that does not rely on pre-patterned microelectronic arrays.
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收藏
页码:8589 / 8594
页数:6
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