Quantitative phase microscopy using defocusing by means of a spatial light modulator

被引：61

作者：

Camacho, Luis ^{[1
]}

Mico, Vicente ^{[1
]}

Zalevsky, Zeev ^{[2
]}

Garcia, Javier ^{[1
]}

机构：

[1] Univ Valencia, Dept Opt, E-46100 Burjassot, Spain

[2] Bar Ilan Univ, Sch Engn, IL-52900 Ramat Gan, Israel

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 07期

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; VOLUME-SPECKLE FIELD; WAVE-FRONT RECONSTRUCTION; TRANSPORT-EQUATION; IMAGE-FORMATION; RETRIEVAL; INTERFEROMETRY; ILLUMINATION; ALGORITHMS; INTENSITY;

D O I：

10.1364/OE.18.006755

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A new method for recovery the quantitative phase information of microscopic samples is presented. It is based on a spatial light modulator (SLM) and digital image processing as key elements to extract the sample's phase distribution. By displaying a set of lenses with different focal power, the SLM produces a set of defocused images of the input sample at the CCD plane. Such recorded images are then numerically processed to retrieve phase information. This iterative process is based on the wave propagation equation and leads on a complex amplitude image containing information of both amplitude and phase distributions of the input sample diffracted wave front. The proposed configuration is a non-interferometric architecture (conventional transmission imaging mode) where no moving elements are included. Experimental results perfectly correlate with the results obtained by conventional digital holographic microscopy (DHM). (C) 2010 Optical Society of America

引用

页码：6755 / 6766

页数：12

共 35 条

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