Pre-, on- and post-column derivatization in capillary electrophoresis

被引:58
作者
Bardelmeijer, HA
Waterval, JCM
Lingeman, H
van't Hof, R
Bult, A
Underberg, WJM
机构
[1] Free Univ Amsterdam, Dept Analyt Chem, NL-1081 HV Amsterdam, Netherlands
[2] Univ Utrecht, Fac Pharm, Dept Pharmaceut Anal, NL-3508 TB Utrecht, Netherlands
关键词
capillary electrophoresis; derivatization; pre-; on-; post-electrophoretic; amino group; reducing group;
D O I
10.1002/elps.1150181212
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This survey gives a short overview of the various reagents and procedures that can be used for pre-, post-and on-column derivatization in capillary electrophoresis. First there is an introduction about capillary electrophoresis as an analytical technique; this is followed by a discussion of the pros and cons of the various modes of derivatization and a comparison with liquid chromatography. In the following paragraphs the reagents for a number of functional groups are discussed. The emphasis is on derivatization of the amino group. Most of the information on the reagents and derivatization procedures is listed in tables together with information on the detection mode, analytes, sensitivity and samples. In addition to the amino group, information is given on labelling of aldehyde, keto, carboxyl, hydroxyl and sulfhydryl groups.
引用
收藏
页码:2214 / 2227
页数:14
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