Oxygen requirements for growth and citric acid production of Yarrowia lipolytica

被引：98

作者：

Kamzolova, SV ^{[1
]}

Shishkanova, NV ^{[1
]}

Morgunov, IG ^{[1
]}

Finogenova, TV ^{[1
]}

机构：

[1] Russian Acad Sci, Lab Aerob Metab Microorganisms, GK Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Region, Russia

来源：

FEMS YEAST RESEARCH | 2003年 / 3卷 / 02期

关键词：

dissolved oxygen; citric acid; iron concentration; Yarrowia lipolytica;

D O I：

10.1016/S1567-1356(02)00188-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

During continuous cultivation of Yarrowia lipolytica N 1, oxygen requirements for growth and citric acid synthesis were found to depend on the iron concentration in the medium. A coupled effect of oxygen and iron concentrations on the functioning of the mitochondrial electron transport chain in Y. lipolytica N 1 was established. Based on the results obtained in continuous culture, conditions for citric acid production in a batch culture of Y. lipolytica N 1 were proposed. At relatively low pO(2) value and a high iron concentration, citric acid accumulation was as high as 120 g l(-1); the specific rate of citric acid synthesis reached 120 mg citric acid (g cells h)(-1). The mass yield coefficient was 0.87 and the energy yield coefficient was 0.31. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：217 / 222

页数：6

共 23 条

[1]

AKIMENKO V K, 1979, Mikrobiologiya, V48, P632

[2]

CITRIC-ACID PRODUCTION FROM GLUCOSE .1. GROWTH AND EXCRETION KINETICS IN A STIRRED FERMENTOR [J].