Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

被引：14

作者：

Botting, CH ^{[1
]}

机构：

[1] Univ St Andrews, Sch Chem, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2003年 / 17卷 / 06期

关键词：

D O I：

10.1002/rcm.954

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and alpha-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：598 / 602

页数：5

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