Strategy for the development of sensor platforms for multi-analysis

Colloidal gold biofunctionalised nanomodules were synthesised and characterised by fluorescence and colorimetric measurements. These biofunctionalised nanomodules were selectively electrodeposited on electrode arrays. When the biomolecule used was an oligonucleotide probe, the gold conjugates were hybridised with complementary and mutated sequences, and the hybridisation efficiency was measured by amperometry. Significant differences in current density between complementary and mutated target sequences were achieved. Horseradish peroxidase-colloidal gold was also selectively electrodeposited on screen-printed electrodes providing proof-of-concept for a construction method of enzyme multi-sensors. Oligonucleotide and enzyme arrays were thus demonstrated, providing a generic platform for use in multi-analysis.