Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham

被引：85

作者：

Jofré, A

Martin, B

Garriga, M

Hugas, M

Pla, M

Rodríguez-Lázaro, D

Aymerich, T

机构：

[1] IRTA, Meat Technol Ctr, E-17121 Monells, Girona, Spain

[2] Univ Girona, INTEA, E-17071 Girona, Spain

来源：

FOOD MICROBIOLOGY | 2005年 / 22卷 / 01期

关键词：

D O I：

10.1016/j.fm.2004.04.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：109 / 115

页数：7

共 38 条

[31] DNA extraction and PCR methods for the detection of Listeria monocytogenes in cold-smoked salmon [J].