Selection of suitable stationary phases and optimum conditions for their application in the separation of basic compounds by reversed-phase HPLC

被引：77

作者：

McCalley, DV ^{[1
]}

机构：

[1] Univ W England, Ctr Biomed Res, Bristol BS16 1QY, Avon, England

来源：

JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 3-4期

关键词：

HPLC; HPLC-MS; column evaluation; basic compounds;

D O I：

10.1002/jssc.200390026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this review, some aspects of the separation of basic compounds by HPLC are discussed with especial reference to work carried out by the author. Procedures for column evaluation are explained, together with the effect of mobile phase modifier, pH, temperature, nature of buffer, and sample overloading on the chromatography of basic compounds. Some evaluations of new column types, such as embedded polar group phases, monoliths, and hybrid inorganic/organic phases are presented.

引用

页码：187 / 200

页数：14

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