Expression of a plant defensin in rice confers resistance to fungal phytopathogens

被引：103

作者：

Jha, Sanjay ^{[1
,2
]}

Chattoo, Bharat B. ^{[1
,2
]}

机构：

[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Ctr Genome Res, Vadodara 390002, Gujarat, India

[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Ctr Biotechnol, Vadodara 390002, Gujarat, India

来源：

TRANSGENIC RESEARCH | 2010年 / 19卷 / 03期

关键词：

Rs-AFP2; Magnaporthe oryzae; Rhizoctonia solani; Transgenic rice; Plant-microbe interactions; SHOWS ENHANCED RESISTANCE; ANTIFUNGAL PROTEINS; VECTORS; OVEREXPRESSION; TRANSFORMATION; AGROBACTERIUM; RADISH; LEVEL; GENE;

D O I：

10.1007/s11248-009-9315-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2 was not accompanied by an induction of pathogenesis-related (PR) gene expression, suggesting that the expression of Rs-AFP2 directly inhibits the pathogens. Here, we demonstrate that transgenic rice plants expressing the Rs-AFP2 gene show enhanced resistance to M. oryzae and R. solani, two of the most important pathogens of rice.

引用

页码：373 / 384

页数：12

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