Lentiviral transgenesis

被引：74

作者：

Pfeifer, A ^{[1
]}

机构：

[1] Univ Munich, Ctr Drug Res, Inst Pharmacol, Dept Pharm, Munich, Germany

来源：

TRANSGENIC RESEARCH | 2004年 / 13卷 / 06期

关键词：

lentiviral vectors; viral gene transfer; transgenic animals; livestock;

D O I：

10.1007/s11248-004-2735-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic animals are relevant for many fields of modern biomedicine and agriculture. However, the inefficiencies of the presently available techniques - DNA microinjection and retroviral gene transfer - have led to an explosion of costs for transgenics especially in farm animals. The recent success in transferring genes to early embryos of different species (mouse, rat, pig, cattle) by viral vectors derived from lentiviruses, has established lentiviral transgenesis as an exciting alternative to the classical method of DNA microinjection. In addition, lentiviral vectors can be used to transfer genes into embryonic stem cells. Due to its high efficacy and versatility, lentiviral transgenesis should have a big impact on transgenic research.

引用

页码：513 / 522

页数：10

共 57 条

[51] Transgenic livestock made easy [J].

Whitelaw, CBA .

TRENDS IN BIOTECHNOLOGY, 2004, 22 (04) :157-159

[52] Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos [J].

Wolfgang, MJ ;

Eisele, SG ;

Browne, MA ;

Schotzko, ML ;

Garthwaite, MA ;

Durning, M ;

Ramezani, A ;

Hawley, RG ;

Thomson, JA ;

Golos, TG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (19) :10728-10732

[53] Transcription start regions in the human genome are favored targets for MLV integration [J].

Wu, XL ;

Li, Y ;

Crise, B ;

Burgess, SM .

SCIENCE, 2003, 300 (5626) :1749-1751

[54] HIV-1 genome nuclear import is mediated by a central DNA flap [J].

Zennou, V ;

Petit, C ;

Guetard, D ;

Nerhbass, U ;

Montagnier, L ;

Charneau, P .

CELL, 2000, 101 (02) :173-185

[55] Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo [J].

Zufferey, R ;

Nagy, D ;

Mandel, RJ ;

Naldini, L ;

Trono, D .

NATURE BIOTECHNOLOGY, 1997, 15 (09) :871-875

[56] Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery [J].

Zufferey, R ;

Dull, T ;

Mandel, RJ ;

Bukovsky, A ;

Quiroz, D ;

Naldini, L ;

Trono, D .

JOURNAL OF VIROLOGY, 1998, 72 (12) :9873-9880

[57] Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors [J].

Zufferey, R ;

Donello, JE ;

Trono, D ;

Hope, TJ .

JOURNAL OF VIROLOGY, 1999, 73 (04) :2886-2892

← 1 2 3 4 5 6 →