Binding of the human Prp31 nop domain to a composite RNA-protein platform in U4 snRNP

被引:109
作者
Liu, Sunbin
Li, Ping
Dybkov, Olexandr
Nottrott, Stephanie
Hartmuth, Klaus
Luehrmann, Reinhard
Carlomagno, Teresa
Wahl, Markus C.
机构
[1] Max Planck Inst Biophys Chem, Abt Zellulaire Biochem, D-37077 Gottingen, Germany
[2] Max Planck Inst Biophys Chem, AG Flussig NMR Spektroskopie, D-37077 Gottingen, Germany
[3] Max Planck Inst Biophys Chem, AG Makromol Rontgenkristallog, D-37077 Gottingen, Germany
关键词
D O I
10.1126/science.1137924
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although highly homologous, the spliceosomal hPrp31 and the nucleolar Nop56 and Nop58 (Nop56/58) proteins recognize different ribonucleoprotein (RNP) particles. hPrp31 interacts with complexes containing the 15.5K protein and U4 or U4atac small nuclear RNA (snRNA), whereas Nop56/58 associate with 15.5K-box C/D small nucleolar RNA complexes. We present structural and biochemical analyses of hPrp31-15.5K-U4 snRNA complexes that show how the conserved Nop domain in hPrp31 maintains high RNP binding selectivity despite relaxed RNA sequence requirements. The Nop domain is a genuine RNP binding module, exhibiting RNA and protein binding surfaces. Yeast two-hybrid analyses suggest a link between retinitis pigmentosa and an aberrant hPrp31-hPrp6 interaction that blocks U4/U6-U5 tri-snRNP formation.
引用
收藏
页码:115 / 120
页数:6
相关论文
共 31 条
[1]   Structure of the S15,S18-rRNA complex: Assembly of the 30S ribosome central domain [J].
Agalarov, SC ;
Prasad, GS ;
Funke, PM ;
Stout, CD ;
Williamson, JR .
SCIENCE, 2000, 288 (5463) :107-112
[2]   Structure and function of archaeal box C/D sRNP core proteins [J].
Aittaleb, M ;
Rashid, R ;
Chen, Q ;
Palmer, JR ;
Daniels, CJ ;
Li, H .
NATURE STRUCTURAL BIOLOGY, 2003, 10 (04) :256-263
[3]   EVIDENCE FOR THE EXISTENCE OF SNRNAS U4 AND U6 IN A SINGLE RIBONUCLEOPROTEIN COMPLEX AND FOR THEIR ASSOCIATION BY INTERMOLECULAR BASE-PAIRING [J].
BRINGMANN, P ;
APPEL, B ;
RINKE, J ;
REUTER, R ;
THEISSEN, H ;
LUHRMANN, R .
EMBO JOURNAL, 1984, 3 (06) :1357-1363
[4]   Allosteric cascade of spliceosome activation [J].
Brow, DA .
ANNUAL REVIEW OF GENETICS, 2002, 36 :333-360
[5]   CONSERVED STRUCTURES AND DIVERSITY OF FUNCTIONS OF RNA-BINDING PROTEINS [J].
BURD, CG ;
DREYFUSS, G .
SCIENCE, 1994, 265 (5172) :615-621
[6]  
Burge CB, 1999, RNA WORLD, P525
[7]   Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis [J].
Gautier, T ;
Berges, T ;
Tollervey, D ;
Hurt, E .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (12) :7088-7098
[8]   Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex [J].
Görnemann, J ;
Kotovic, KM ;
Hujer, K ;
Neugebauer, KM .
MOLECULAR CELL, 2005, 19 (01) :53-63
[9]   U4 AND U6 RNAS COEXIST IN A SINGLE SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE [J].
HASHIMOTO, C ;
STEITZ, JA .
NUCLEIC ACIDS RESEARCH, 1984, 12 (07) :3283-3293
[10]   Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles [J].
Kühn-Hölsken, E ;
Lenz, C ;
Sander, B ;
Lührmann, R ;
Urlaub, H .
RNA, 2005, 11 (12) :1915-1930