Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR)

被引：1044

作者：

Kroh, Evan M. ^{[1
]}

Parkin, Rachael K. ^{[1
]}

Mitchell, Patrick S. ^{[1
]}

Tewari, Muneesh ^{[1
,2
,3
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98104 USA

[2] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98104 USA

[3] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA

来源：

METHODS | 2010年 / 50卷 / 04期

关键词：

microRNA; Biomarker; Circulating; qRT-PCR; miRNA; QUANTIFICATION;

D O I：

10.1016/j.ymeth.2010.01.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are small (similar to 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measurement of circulating miRNAs as biomarkers is associated with some special challenges, including those related to pre-analytic variation and data normalization. We describe here our procedure for qRT-PCR analysis of circulating miRNAs as biomarkers, and discuss relevant issues of sample preparation, experimental design and data analysis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：298 / 301

页数：4

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