Determination of uric acid in human saliva and urine using miniaturized capillary electrophoresis with amperometric detection

被引：28

作者：

Chu, Q. C. ^{[1
]}

Lin, M. ^{[1
]}

Geng, C. H. ^{[1
]}

Ye, J. N. ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

CHROMATOGRAPHIA | 2007年 / 65卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; amperometric detection; miniaturization; uric acid in human saliva and urine;

D O I：

10.1365/s10337-006-0142-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method based on a novel miniaturized capillary electrophoresis with amperometric detection (CE-AD) system was described to study the correlation between the trace amount of uric acid (UA) in human saliva and urine samples for patients suffering from gout disease. Under the optimum conditions, UA can been well separated from its co-existing potential interferences, namely xanthine, hypoxanthine, and ascorbic acid, within 170 s with estimated separation efficiency of 111,210 TP m(-1). The proposed miniaturized CE-AD system has been successfully applied for the analysis of UA in less invasive biological samples, which could provide potential application in fast diagnosis of gout.

引用

页码：179 / 184

页数：6

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