A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae

被引：199

作者：

Imperi, Monica ^{[1
]}

Pataracchia, Marco ^{[1
]}

Alfarone, Giovanna ^{[1
]}

Baldassarri, Lucilla ^{[1
]}

Orefici, Graziella ^{[1
]}

Creti, Roberta ^{[1
]}

机构：

[1] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 80卷 / 02期

关键词：

Group B streptococcus; S; agalactiae; Multiplex PCR; Capsular operon; Serotype; GROUP-B STREPTOCOCCUS; SEROTYPE IDENTIFICATION; SURFACE-PROTEINS; INFECTIONS; COLONIZATION; RESISTANCE; DIVERSITY; INFANTS; REGION; WOMEN;

D O I：

10.1016/j.mimet.2009.11.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：212 / 214

页数：3

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