Phenotyping transgenic embryonic murine hearts using optical coherence tomography

被引：33

作者：

Jenkins, Michael W.

Patel, Pankti

Deng, Huayun

Montano, Monica M.

Watanabe, Michiko

Rollins, Andrew M.

机构：

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Sch Med, Dept Pediat, Cleveland, OH 44106 USA

来源：

APPLIED OPTICS | 2007年 / 46卷 / 10期

关键词：

BREAST CELL-GROWTH; INHIBITOR;

D O I：

10.1364/AO.46.001776

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We used optical coherence tomography (OCT) to characterize the morphological phenotype of embryonic murine hearts discerning hexamethylene-bis-acetamide-inducible protein I (HEXIM1) mutants from their wild-type littermates. At E12.5 and E13.5 murine embryos were excised from the mother, the hearts were removed, and 3D OCT data sets were obtained from each heart in the litter. Next, we segmented the morphological borders to obtain cavity volumes and wall thicknesses. The mutant hearts exhibited increased ventricular chamber volume and decreased compact myocardium wall thickness when compared with their wild-type littermates. Also, the E13.5 HEXIM1 -/- embryo was distinguished by morphological asymmetry (underdeveloped left side). (c) 2007 Optical Society of America.

引用

页码：1776 / 1781

页数：6

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