Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE

被引：67

作者：

Kitagawa, M ^{[1
]}

Umezu, M ^{[1
]}

Aoki, J ^{[1
]}

Koizumi, H ^{[1
]}

Arai, H ^{[1
]}

Inoue, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

FEBS LETTERS | 2000年 / 479卷 / 1-2期

关键词：

LIS1; platelet-activating factor; platelet-activating factor acetylhydrolase; NUDF; NUDE;

D O I：

10.1016/S0014-5793(00)01856-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LIS1 is a product of the causative gene for type I lissencephaly characterized by a smooth brain surface due to a defect in neuronal migration during brain development and a regulatory subunit of platelet-activating factor acetylhydrolase (PAF-AH). It is also a mammalian homologue of the fungal nuclear distribution (nud) gene, nudF, which controls the migration of fungal nuclei. Using the two-hybrid system, we identified a novel LIS1-interacting protein, rat NUDE (rNUDE), and found that it is a mammalian homologue of another fungal nud gene product, NUDE, and Xenopus mitotic phosphoprotein 43 which is phosphorylated in a cell cycle-dependent manner, rNUDE and the catalytic subunits of PAF-AH interact with the N- and C-termini of LIS1, respectively. However, these proteins, instead of simultaneously binding to LIS1, appeared to bind to LIS1 in a competitive manner. These results suggest that LIS1 functions in nuclear migration by interacting with multiple intracellular proteins in mammals. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：57 / 62

页数：6

共 28 条

[11]

Liu Z, 1999, DEVELOPMENT, V126, P4477

[12]

LO NC, 1997, HUM MOL GENET, V6, P157

[13] Switching of platelet-activating factor acetylhydrolase catalytic subunits in developing rat brain [J].

Manya, H ;

Aoki, J ;

Watanabe, M ;

Adachi, T ;

Asou, H ;

Inoue, Y ;

Arai, H ;

Inoue, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (29) :18567-18572

[14] Biochemical characterization of various catalytic complexes of the brain platelet-activating factor acetylhydrolase [J].

Manya, H ;

Aoki, J ;

Kato, H ;

Ishii, J ;

Hino, S ;

Arai, H ;

Inoue, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (45) :31827-31832

[15] Neurospora crassa ro-10 and ro-11 genes encode novel proteins required for nuclear distribution [J].

Minke, PF ;

Lee, IH ;

Tinsley, JH ;

Bruno, KS ;

Plamann, M .

MOLECULAR MICROBIOLOGY, 1999, 32 (05) :1065-1076

[16] Nuclear migration, nucleokinesis and lissencephaly [J].

Morris, NR ;

Efimov, VP ;

Xiang, X .

TRENDS IN CELL BIOLOGY, 1998, 8 (12) :467-470

[17]

REINER O, 1995, J NEUROSCI, V15, P3730

[18] LIS2, GENE AND PSEUDOGENE, HOMOLOGOUS TO LIS1 (LISSENCEPHALY-1), LOCATED ON THE SHORT AND LONG ARMS OF CHROMOSOME-2 [J].

REINER, O ;

BARAM, I ;

SAPIR, T ;

SHMUELI, O ;

CARROZZO, R ;

LINDSAY, EA ;

BALDINI, A ;

LEDBETTER, DH ;

CAHANA, A .

GENOMICS, 1995, 30 (02) :251-256

[19] ISOLATION OF A MILLER-DIEKER LISSENCEPHALY GENE CONTAINING G-PROTEIN BETA-SUBUNIT-LIKE REPEATS [J].

REINER, O ;

CARROZZO, R ;

SHEN, Y ;

WEHNERT, M ;

FAUSTINELLA, F ;

DOBYNS, WB ;

CASKEY, CT ;

LEDBETTER, DH .

NATURE, 1993, 364 (6439) :717-721

[20] LIS1 is a microtubule-associated phosphoprotein [J].

Sapir, T ;

Cahana, A ;

Seger, R ;

Nekhai, S ;

Reiner, O .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 265 (01) :181-188

← 1 2 3 →