Biological Activity of a Cholesterol Modified Fullerene/γ-Cyclodextrin Inclusion Complex

被引：9

作者：

Gao Yun-Yan ^{[1
]}

Liu Li-Hua ^{[1
]}

Ou Zhi-Ze ^{[1
]}

Li Yi ^{[2
]}

Yang Guo-Qiang ^{[3
]}

Wang Xue-Song ^{[2
]}

机构：

[1] Northwestern Polytech Univ, Dept Appl Chem, Sch Sci, Xian 710072, Peoples R China

[2] Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100190, Peoples R China

[3] Chinese Acad Sci, Key Lab Photochem, Inst Chem, Beijing 100190, Peoples R China

来源：

ACTA PHYSICO-CHIMICA SINICA | 2010年 / 26卷 / 02期

关键词：

Fullerene; Cyclodextrin inclusion complex; Serum albumin; DNA cleavage; PHOTOINDUCED ELECTRON-TRANSFER; METHYLENE-COMPOUNDS; WATER; C-60; ALBUMIN; BINDING; PHOTOCLEAVAGE; FLUORESCENCE; HYPOCRELLIN; LIPOSOMES;

D O I：

10.3866/PKU.WHXB20100238

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A cholesterol modified fullerene (CHL-C-60) was synthesized using the Bingel-Hirsch reaction and was characterized by nuclear magnetic resonance (NMR), mass spectra (MS), elemental analysis (EA). Results of UV-Vis spectrum and fluorescence spectrum indicate that CHL-C-60 forms an inclusion complex with gamma-cyclodextrin (gamma-CD) due to the strong interaction between gamma-cyclodextrin and the steroid ring. The water solubility of CHL-C-60 is enhanced significantly. CHL-C-60 can dissociate from cyclodextrin and subsequently bind to human serum albumin (HSA) and bovine serum albumin (BSA) with association constants of 5.73 x 10(4) and 7.05 x 10(4) L.moL(-1), respectively. The efficiency of desoxyribonucleic acid (DNA) photocleavage for CHL-C-omega/gamma-CD is as high as 60.5% under anaerobic conditions, which is caused by photoinduced electron transfer from pBR322 plasmid DNA to CHL-C-60.

引用

页码：495 / 501

页数：7

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