Peak compression effects in packed-column supercritical fluid chromatography

被引：10

作者：

Strode, JT ^{[1
]}

Gyllenhaal, O ^{[1
]}

Torstensson, A ^{[1
]}

Karlsson, A ^{[1
]}

Karlsson, L ^{[1
]}

机构：

[1] Astra Hassle AB, Pharmaceut Res & Dev, S-43183 Molndal, Sweden

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1998年 / 36卷 / 05期

关键词：

D O I：

10.1093/chromsci/36.5.257

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peak compression phenomena are studied and applied to the analysis of lipid emulsion samples of clevidipine. Alcohols and water are found to generate system peaks on a silica column with 2-propanol-modified carbon dioxide. The retention times of the system peaks are found to vary as a function of type (water or alcohol) and chromatographic conditions (pressure, temperature, and modifier). By selecting an appropriate system peak generator and chromatographic conditions, the peak compression effect is created for the analysis of an emulsion sample of clevidipine solution containing water, methanol, and acetonitrile. The presence of buffer or lipids in the sample does not affect the peak compression phenomena.

引用

页码：257 / 262

页数：6

共 16 条

[1] CHIRAL SEPARATIONS BY PACKED-COLUMN SUPER-CRITICAL AND SUBCRITICAL FLUID CHROMATOGRAPHY [J].

ANTON, K ;

EPPINGER, J ;

FREDERIKSEN, L ;

FRANCOTTE, E ;

BERGER, TA ;

WILSON, WH .

JOURNAL OF CHROMATOGRAPHY A, 1994, 666 (1-2) :395-401

[2] SEPARATION OF DRUGS BY PACKED-COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY .1. PHENOTHIAZINE ANTIPSYCHOTICS [J].

BERGER, TA ;

WILSON, WH .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1994, 83 (03) :281-286

[3] SEPARATION OF DRUGS BY PACKED-COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY .2. ANTIDEPRESSANTS [J].

BERGER, TA ;

WILSON, WH .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1994, 83 (03) :287-290

[4]

BERGER TA, 1996, RCS CHROMATOGRAPHY M

[5]

Box G.E.P., 1978, STATISTICS EXPT, P306

[6] Investigation of peak compression effects in packed column supercritical fluid chromatography using chemometrics [J].

Carlsson, D ;

Strode, JT ;

Gyllenhaal, O ;

Karlsson, A ;

Karlsson, L .

CHROMATOGRAPHIA, 1997, 44 (5-6) :289-294

[7] STUDIES ON SYSTEM PEAKS IN ION-PAIR ADSORPTION CHROMATOGRAPHY .3. REGULATION OF SYSTEM PEAK GRADIENT RETENTION FOR OBTAINING ANALYTE PEAK COMPRESSION [J].

FORNSTEDT, T ;

WESTERLUND, D ;

SOKOLOWSKI, A .

JOURNAL OF CHROMATOGRAPHY, 1990, 506 :61-74

[8] STUDIES ON SYSTEM PEAKS IN ION-PAIR ADSORPTION CHROMATOGRAPHY .2. EFFECTS ON ANALYTE PEAK COMPRESSION AND DEFORMATION [J].

FORNSTEDT, T ;

WESTERLUND, D ;

SOKOLOWSKI, A .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (13) :2645-2684

[9] PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY OF OMEPRAZOLE AND RELATED-COMPOUNDS - SELECTION OF COLUMN SUPPORT WITH TRIETHYLAMINE-MODIFIED AND METHANOL-MODIFIED CARBON-DIOXIDE AS THE MOBILE PHASE [J].

GYLLENHAAL, O ;

VESSMAN, J .

JOURNAL OF CHROMATOGRAPHY, 1993, 628 (02) :275-281

[10]

GYLLENHAAL O, 1995, 6 INT S AN SFC SFE S

← 1 2 →