An Amperomertic Uric Acid Biosensor Based on Immobilization of Uricase onto Polyaniline-multiwalled Carbon Nanotube Composite Film

被引：44

作者：

Bhambi, Manu ^{[1
]}

Sumana, G. ^{[2
]}

Malhotra, B. D. ^{[2
]}

Pundir, C. S. ^{[1
]}

机构：

[1] MD Univ, Dept Biochem & Genet, Biochem Res Lab, Rohtak 124001, Haryana, India

[2] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2010年 / 38卷 / 04期

关键词：

uric acid; biosensor; multiwalled carbon nanotubes; biocomposites; polyaniline; uricase; serum; DIRECT ELECTRON-TRANSFER; MODIFIED GOLD ELECTRODE; POLYPYRROLE FILM; ASCORBIC-ACID; MEMBRANE; SERUM; PERFORMANCE; LIQUID;

D O I：

10.3109/10731191003716344

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly sensitive, amperometric uric acid biosensor possessing unique physical, elcctro-conductive properties of carbon nanotubes is described for determination of uric acid level in scrum. A uric acid biosensor was constructed after immobilization of unease onto a polyaniline/CNT matrix by carbonamide linkage. The scanning electron micrographs confirmed the immobilization of globular enzyme onto the electroploymerized polyaniline/CNT composite. Employment of carbon nanotubes composite in the present electrode leads to a very quick response time of 8 sec with the minimum detection limit of 5 mu M along with an increase in shelf-life of electrode system to >180 days with slight loss of enzyme activity. Efficiency of the present amperometric uric acid biosensor was validated by quantitative estimation of uric acid level in biological scrum of healthy individuals and the persons suffering from hyperuricemia and gout.

引用

页码：178 / 185

页数：8

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