ORFeomics: correcting the wiggle in worm genes

被引：3

作者：

Boone, C

Andrews, B

机构：

[1] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada

来源：

NATURE GENETICS | 2003年 / 34卷 / 01期

关键词：

D O I：

10.1038/ng0503-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new study attempts to amplify and clone all the predicted protein-encoding open reading frames (ORFs) for Caenorhabditis elegans. This analysis confirms many of the predicted genes but suggests roughly 50% of them require correction. Recombining the ORFs into a number of different expression systems can generate functional proteomics kits for characterizing protein activity and interaction networks.

引用

页码：8 / 9

页数：2

共 13 条

[1]

*C EL SEQ CONS, 1998, SCIENCE, V282, P2102

[2] Systematic functional analysis of the Caenorhabditis elegans genome using RNAi [J].

Kamath, RS ;

Fraser, AG ;

Dong, Y ;

Poulin, G ;

Durbin, R ;

Gotta, M ;

Kanapin, A ;

Le Bot, N ;

Moreno, S ;

Sohrmann, M ;

Welchman, DP ;

Zipperlen, P ;

Ahringer, J .

NATURE, 2003, 421 (6920) :231-237

[3] Protein complexes take the bait [J].

Kumar, A ;

Snyder, M .

NATURE, 2002, 415 (6868) :123-124

[4] Emerging technologies in yeast genomics [J].

Kumar, A ;

Snyder, M .

NATURE REVIEWS GENETICS, 2001, 2 (04) :302-312

[5] Current methods of gene prediction, their strengths and weaknesses [J].

Mathé, C ;

Sagot, MF ;

Schiex, T ;

Rouzé, P .

NUCLEIC ACIDS RESEARCH, 2002, 30 (19) :4103-4117

[6] Protein analysis on a proteomic scale [J].

Phizicky, E ;

Bastiaens, PIH ;

Zhu, H ;

Snyder, M ;

Fields, S .

NATURE, 2003, 422 (6928) :208-215

[7] C-elegans ORFeome version 1.1:: experimental verification of the genome annotation and resource for proteome-scale protein expression [J].

Reboul, J ;

Vaglio, P ;

Rual, JF ;

Lamesch, P ;

Martinez, M ;

Armstrong, CM ;

Li, SM ;

Jacotot, L ;

Bertin, N ;

Janky, R ;

Moore, T ;

Hudson, JR ;

Hartley, JL ;

Brasch, MA ;

Vandenhaute, J ;

Boulton, S ;

Endress, GA ;

Jenna, S ;

Chevet, E ;

Papasotiropoulos, V ;

Tolias, PP ;

Ptacek, J ;

Snyder, M ;

Huang, R ;

Chance, MR ;

Lee, HM ;

Doucette-Stamm, L ;

Hill, DE ;

Vidal, M .

NATURE GENETICS, 2003, 34 (01) :35-41

[8] Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C-elegans [J].

Reboul, J ;

Vaglio, P ;

Tzellas, N ;

Thierry-Mieg, N ;

Moore, T ;

Jackson, C ;

Shin-i, T ;

Kohara, Y ;

Thierry-Mieg, D ;

Thierry-Mieg, J ;

Lee, H ;

Hitti, J ;

Doucette-Stamm, L ;

Hartley, JL ;

Temple, GF ;

Brasch, MA ;

Vandenhaute, J ;

Lamesch, PE ;

Hill, DE ;

Vidal, M .

NATURE GENETICS, 2001, 27 (03) :332-336

[9] Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans [J].

Roy, PJ ;

Stuart, JM ;

Lund, J ;

Kim, SK .

NATURE, 2002, 418 (6901) :975-979

[10] Experimental annotation of the human genome using microarray technology [J].

Shoemaker, DD ;

Schadt, EE ;

Armour, CD ;

He, YD ;

Garrett-Engele, P ;

McDonagh, PD ;

Loerch, PM ;

Leonardson, A ;

Lum, PY ;

Cavet, G ;

Wu, LF ;

Altschuler, SJ ;

Edwards, S ;

King, J ;

Tsang, JS ;

Schimmack, G ;

Schelter, JM ;

Koch, J ;

Ziman, M ;

Marton, MJ ;

Li, B ;

Cundiff, P ;

Ward, T ;

Castle, J ;

Krolewski, M ;

Meyer, MR ;

Mao, M ;

Burchard, J ;

Kidd, MJ ;

Dai, H ;

Phillips, JW ;

Linsley, PS ;

Stoughton, R ;

Scherer, S ;

Boguski, MS .

NATURE, 2001, 409 (6822) :922-+

← 1 2 →