DNA and low titer, helper-free, recombinant AAV prime-boost vaccination for cytomegalovirus induces an immune response to CMV-pp65 and CMV-IE1 in transgenic HLA A*0201 mice

被引：18

作者：

Gallez-Hawkins, G

Li, XL

Franck, AE

Thao, L

Lacey, SF

Diamond, DJ

Zaia, JA ^{[1
]}

机构：

[1] City Hope Natl Med Ctr, Beckman Res Inst, Dept Virol, Duarte, CA 91010 USA

[2] City Hope Natl Med Ctr, Beckman Res Inst, Lab Vaccine Res, Duarte, CA 91010 USA

来源：

VACCINE | 2004年 / 23卷 / 06期

关键词：

cytomegalovirus; RAW; immunization;

D O I：

10.1016/j.vaccine.2004.06.048

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A prime-boost immunization regimen allowed the use of low titer, helper-free rAAV-pp65mII and rAAV-IE1 virus to elicit-specific humoral cellular responses to two important cytomegalovirus (CMV) antigens: the immediate-early I (IE-1) and pp65 proteins. Simultaneous immunization of both CMV proteins, using DNA vaccine priming followed by rAAV boost, induced antibody (Ab) response. CD8 lymphocytes with cytotoxic function. and detectible binding of the cognate peptide epitopes for human HLA A*0201 restriction using tetramer technology. 2004 Elsevier Ltd. All rights reserved.

引用

收藏

页码：819 / 826

页数：8

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