学术探索
学术期刊
新闻热点
数据分析
智能评审

RNA interference: potential therapeutic targets

被引：43
作者：
Jana, S
Chakraborty, C
Nandi, S
Deb, JK
机构：
[1] Inst Appl Med & Res, Dept Biotechnol, Duhai, Ghaziabad, India
[2] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2004年 / 65卷 / 06期
关键词：
D O I：
10.1007/s00253-004-1732-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
One of the most exciting findings in recent years has been the discovery of RNA interference (RNAi). RNAi methodologies hold the promise to selectively inhibit gene expression in mammals. RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences, including endogenous mRNAs. The use of RNAi for genetic-based therapies has been widely studied, especially in viral infections, cancers, and inherited genetic disorders. As such, RNAi technology is a potentially useful method to develop highly specific dsRNA-based gene-silencing therapeutics.
引用
收藏
页码：649 / 657
页数：9
相关论文
共 91 条
[11]   Rescue of polyglutamine-mediated cytotoxicity by double-stranded RNA-mediated RNA interference [J].
Caplen, NJ ;
Taylor, JP ;
Statham, VS ;
Tanaka, F ;
Fire, A ;
Morgan, RA .
HUMAN MOLECULAR GENETICS, 2002, 11 (02) :175-184
[12]   A new approach to the inhibition of gene expression [J].
Caplen, NJ .
TRENDS IN BIOTECHNOLOGY, 2002, 20 (02) :49-51
[13]   Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J].
Caplen, NJ ;
Parrish, S ;
Imani, F ;
Fire, A ;
Morgan, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9742-9747
[14]   RNA interference: traveling in the cell and gaining functions? [J].
Cerutti, H .
TRENDS IN GENETICS, 2003, 19 (01) :39-46
[15]   Molecular medicine for the brain: silencing of disease genes with RNA interference [J].
Davidson, BL ;
Paulson, HL .
LANCET NEUROLOGY, 2004, 3 (03) :145-149
[16]   RNAi: an ever-growing puzzle [J].
Denli, AM ;
Hannon, GJ .
TRENDS IN BIOCHEMICAL SCIENCES, 2003, 28 (04) :196-201
[17]   The specifics of small interfering RNA specificity [J].
Dillin, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6289-6291
[18]   RNA interference is mediated by 21-and 22-nucleotide RNAs [J].
Elbashir, SM ;
Lendeckel, W ;
Tuschl, T .
GENES & DEVELOPMENT, 2001, 15 (02) :188-200
[19]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[20]   Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate [J].
Elbashir, SM ;
Martinez, J ;
Patkaniowska, A ;
Lendeckel, W ;
Tuschl, T .
EMBO JOURNAL, 2001, 20 (23) :6877-6888
12345678910