An amperometric urea biosensor based on covalent immobilization of urease onto an electrochemically prepared copolymer poly (N-3-aminopropyl pyrrole-co-pyrrole) film

被引：169

作者：

Rajesh ^{[1
]}

Bisht, V ^{[1
]}

Takashima, W ^{[1
]}

Kaneto, K ^{[1
]}

机构：

[1] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Wakamatsu Ku, Kitakyushu, Fukuoka 8080196, Japan

来源：

BIOMATERIALS | 2005年 / 26卷 / 17期

基金：

日本学术振兴会;

关键词：

urea; polymerization; biosensor; covalent immobilization; peptide linkage;

D O I：

10.1016/j.biomaterials.2004.09.024

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

An amperometric biosensor has been developed for the quantitative determination of urea in aqueous solution. The principle is based on the use of pH-sensitive redox active dissolved hematein molecule. The enzyme, urease (Urs), was covalently immobilized on a conducting copolymer poly (N-3-aminopropyl pyrrole-co-pyrrole) film, electrochemically prepared onto an indium-tin-oxide (ITO)-coated glass plate. The covalent linkage of enzyme and porous morphology of the polymer film lead to high enzyme loading and an increased lifetime stability of the enzyme electrode. Amperometric response was measured as a function of concentration of urea, at fixed bias voltage of 0.0 V vs. Ag/AgCl in a phosphate buffer (pH 7.0). The electrode gives a linear response range of 0.16-5.02 mm for urea in aqueous medium. The response time is 40 s reaching to a 95% steady-state current value, and 80% of the enzyme activity is retained for about 2 months. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3683 / 3690

页数：8

共 37 条

[11] Infrared reflection absorption and sum frequency generation spectroscopic study on the structures of the LB films of palmitoyl-L- and DL-ornithine and palmitoyl-L- and DL-lysine [J].