The Complete Genome Sequence of Haloferax volcanii DS2, a Model Archaeon

被引：195

作者：

Hartman, Amber L. ^{[1
,2
,9
]}

Norais, Cedric ^{[3
,11
]}

Badger, Jonathan H. ^{[2
]}

Delmas, Stephane ^{[7
]}

Haldenby, Sam ^{[7
]}

Madupu, Ramana ^{[2
]}

Robinson, Jeffrey ^{[2
]}

Khouri, Hoda ^{[2
]}

Ren, Qinghu ^{[2
]}

Lowe, Todd M. ^{[4
]}

Maupin-Furlow, Julie ^{[5
]}

Pohlschroder, Mecky ^{[6
]}

Daniels, Charles ^{[8
]}

Pfeiffer, Friedhelm ^{[10
]}

Allers, Thorsten ^{[7
]}

Eisen, Jonathan A. ^{[2
,9
,12
,13
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[2] J Craig Venter Inst, Inst Genom Res, Rockville, MD USA

[3] Univ Paris Sud, Inst Genet & Microbiol, Paris, France

[4] Univ Calif Santa Cruz, Dept Biomol Engn, Santa Cruz, CA 95064 USA

[5] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[6] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

[7] Univ Nottingham, Inst Genet, Nottingham NG7 2RD, England

[8] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

[9] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA

[10] Max Planck Inst Biochem, Dept Membrane Biochem, D-82152 Martinsried, Germany

[11] Univ Wisconsin, Dept Biochem, Madison, WI 53705 USA

[12] Univ Calif Davis, Dept Med Microbiol & Immunol, Davis, CA 95616 USA

[13] Univ Calif Davis, Dept Ecol & Evolut, Davis, CA 95616 USA

来源：

PLOS ONE | 2010年 / 5卷 / 03期

基金：

美国国家科学基金会;

关键词：

HALOPHILIC ARCHAEON; HALOBACTERIUM-HALOBIUM; RNA POLYADENYLATION; SIGNAL-TRANSDUCTION; PYROCOCCUS-ABYSSI; ESCHERICHIA-COLI; MISMATCH-REPAIR; SHUTTLE VECTORS; GENE-TRANSFER; DNA;

D O I：

10.1371/journal.pone.0009605

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general. Methodology/Principal Findings: We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb). Conclusions/Significance: The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.

引用

页数：20

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